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GEt chimera is partially processed by late Golgi enzymes. BHK cells expressing wild-type G or GEt were pulse-labeled with [35S]methionine-cysteine, chased for 0, 15, or 30 min, lysed, and immunoprecipitated with anti-VSV antibody. The immunoprecipitates were mock treated (lanes −) or treated with endo H (lanes +), and subjected to SDS-10% PAGE and fluorography to determine the extent of endo H resistance and sialylation.
