FIG. 8.

IBV E and CTE are localized with Golgi remnants after BFA treatment. BHK cells expressing E or CTE by infection with recombinant vaccinia viruses were treated with BFA for 2 h at 3 and 5 h postinfection, respectively, fixed for immunofluorescence, permeabilized, and double labeled with rabbit anti-E antibody and anti-GM130 antibody or mouse anti-p115 antibody, or rat anti-E antibody and rabbit anti-mannosidase II (manII) antibody as indicated. Secondary antibodies were fluorescein-conjugated donkey anti-rabbit IgG and Texas Red-conjugated goat anti-mouse IgG, or fluorescein-conjugated goat anti-rat IgG and Texas Red-conjugated goat anti-mouse IgG. Boxed regions are enlarged in the insets of the BFA-treated panels. Bar, 10 μm.