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. 2026 Sep 4;7:1956432. doi: 10.3389/froh.2026.1956432

Table 1.

Emerging directions for ecological prevention and translational research in early childhood caries.

Future area Scientific rationale Research and translational implications Key references
Preconception oral ecosystem optimization Maternal microbial communities contribute to early microbial transmission and may influence oral ecosystem assembly. Integration of oral health into preconception and prenatal care; investigation of maternal ecological determinants of offspring oral health. (14, 126, 132)
Ecologically designed infant nutrition Human milk bioactive components influence microbial colonization, host–microbe interactions, and immune maturation. Development of microbiome-targeted nutritional strategies, including HMOs and postbiotic-enriched formulations when breastfeeding is not possible. (35, 133)
Ecological biomarkers and functional risk assessment Microbial resilience, community interactions, and metabolic functions may better reflect disease susceptibility than individual taxa. Development of ecological biomarkers and functional microbiome profiling tools for personalized caries risk assessment. (7, 8, 86)
Functional biofilm modulation Biofilm ecology may be influenced by targeting microbial functions, communication pathways, and ecological interactions rather than indiscriminate microbial elimination. Investigation of postbiotics, bacteriocins, quorum-sensing inhibitors, and microbiome-directed therapies for ecological biofilm management. (134, 135)
Longitudinal ecological research across the first 1,000 days Oral ecosystem development is a dynamic process shaped by biological, nutritional, behavioral, and environmental exposures. Prospective birth cohorts integrating microbial, nutritional, behavioral, and environmental data to identify critical developmental windows and validate ecological biomarkers. (14, 17, 119)
Precision ecological prevention Integration of developmental biology, oral microbiomics, and ecological risk assessment may support individualized prevention strategies. Development of personalized prevention programs based on ecological profiles, developmental trajectories, and ecosystem resilience. (8, 86, 115)

The developmental-ecological framework proposed in this review supports a shift from disease-centered prevention toward strategies aimed at promoting oral ecosystem development, resilience, and long-term ecological stability. The table summarizes the main future research areas and their potential translational applications.