FIG. 2.

Rosco inhibits ICP0-induced expression of β-gal from the ICP6 promoter. (A) Experimental design. Vero cells were pretreated with CHX (50 μg/ml) for 1 h (t = −1) and mock infected or infected with 5 PFU of KOS6β per cell at t = 0 in the presence of CHX for 6 h (t = 6 hpi). Cells were then either harvested or released into medium with or without CHX (50 μg/ml), ActD (1 μg/ml), Rosco (100 μM), or PAA (100 μg/ml) for an additional 6 h. At the end of the 6-h treatment period (t = 12 hpi), cell extracts were prepared and β-gal assays were performed. (B) Histogram showing results of a typical β-gal assay. All samples were compared to mock-infected cells as controls (far left sample); this control was given the arbitrary value of “1.” The data shown represent the means of duplicate samples; bars indicate the standard deviations of the means.