FIG. 5.
Rosco affects the electrophoretic mobility of ICP0. (A) Experimental design. Vero cells were pretreated with CHX (50 μg/ml) for 1 h (t = −1) and mock infected or infected with 5 PFU of KOS per cell (t = 0 hpi) and incubated in the presence of CHX for 6 h (t = 6 hpi). Cells were released from the CHX block into methionine-free medium containing [35S]methionine in the absence or presence of Rosco (100 μM) for an additional 6 h (t = 12 hpi). At the end of the second 6 h (t = 12 hpi), extracts were prepared and proteins were resolved by SDS-PAGE and transferred to nitrocellulose. (B) SDS-PAGE analysis. Samples from mock-infected cells (MI), control cells infected with KOS in the absence of Rosco (C), and cells infected with KOS in the presence of Rosco (RO) were resolved by SDS-6% PAGE. Molecular mass markers (in kilodaltons) and the band specific for ICP0 (large arrow) are indicated on the left. Radiolabeled bands were visualized by PhosphorImager analysis.