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. 2002 Feb;76(3):1025–1032. doi: 10.1128/JVI.76.3.1025-1032.2002

FIG. 7.

FIG. 7.

Immunoblots of the sucrose gradient sedimentations of wild-type EBNA-LPR4(F) and EBNA-LPR4RA(F). BJAB cells were transfected with the expression vector for EBNA-LPR4(F) or EBNA-LPR4RA(F), harvested 1 day after transfection, solubilized, and applied to a 10-to-30% sucrose gradient. The gradient was run for 16 h, and then samples were collected from the top as 35 0.3-ml fractions, subjected to electrophoresis in denaturing gels, transferred to nitrocellulose sheets, and reacted with anti-EBNA-LP monoclonal antibody. Lane 11, 10% of whole-cell extract (WCE) used in the sucrose gradient sedimentations. Positions of molecular mass markers are on the right.