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(A) Rv minigenome constructions. Leader and trailer sequences are represented reversed because all of the constructions encode a negative-strand Rv minigenome. The RNA produced after transcription and ribozyme cleavage is shown protruding. (B) Ribozyme sequences and secondary structures are shown flanking a negative-strand minigenome in which the luciferase gene has been cloned. The gray boxes indicate the nucleotides mutated in the HdvRz to insert an SmaI restriction site.
