FIG. 4.

(A) T3SA⁺ protein synthesis in cells treated with ribavirin. HeLa cells (2 × 10⁶) were either mock infected or infected with T3SA⁺ at an MOI of 1,000 PFU per cell. Cells were incubated for 24 h in the presence of ribavirin (Rib) at the concentrations shown and [³⁵S]methionine-cysteine. Viral proteins were immunoprecipitated from cell lysates, resolved by SDS-PAGE, dried, and exposed to film. Reovirus λ, μ, and ς proteins are indicated. Total viral protein was quantitated by phosphorimager analysis, and the relative amount of protein present is indicated (protein in untreated cells is arbitrarily set at 100). (B and C) Apoptosis induced by reovirus (B) or TNF-α (C) in cells treated with ribavirin. HeLa cells (5 × 10⁴) were mock infected, infected with T3SA⁺ at an MOI of 1,000 PFU per cell, or treated with 10 ng of TNF-α per ml and incubated in the absence or presence of ribavirin at the concentrations shown. Mock-infected cells (B) and ribavirin-treated cells (C) were incubated in the presence of 200 μM ribavirin. After incubation for 24 h, cells were stained with acridine orange. The results are expressed as the mean percentage of cells undergoing apoptosis for three independent experiments. Error bars indicate standard deviations of the means.