TABLE 1.

Characteristics of reovirus ts mutants^a

Mutant	Gene	Protein	Replication block	EOY (39°C)b
tsA201	M2	μ1	Entry of ts progeny	0.028
tsB352	L2	λ2	Assembly of outer capsid	0.000027
tsC447	S2	ς2	Assembly of core particles, dsRNA synthesis	0.00012
tsD357	L1	λ3	dsRNA synthesis	0.0001
tsE320	S3	ςNS	dsRNA synthesis	0.00014
tsG453	S4	ς3	Assembly of outer capsid	0.00014

^aReovirus ts mutants were generated by chemical mutagenesis of strain T3D (20, 26). Gene segment assignments and replication blocks of each ts mutant have been previously reported (14, 20, 21, 22, 26, 27).

^bThe viral yield of each ts mutant was determined after 48 h of growth in HeLa cells incubated at either 32 or 39°C. EOY was calculated by dividing viral yield at 39°C by viral yield at 32°C.