FIG. 3.

pAb 5-6-precipitable processing products derived from nsP1a expressed in BHK cells or Caco-2 cells. nsP1a was expressed in BHK cells as described in the legend to Fig. 1A, except that labeling was followed by an 85-min cold chase. Caco-2 cells in six-well plates were infected with astrovirus at a multiplicity of infection of 4 or were mock infected. At 4 h postinfection, the cells were starved for 30 min in medium without Cys or Met and then metabolically labeled with 50 μCi of [³⁵S]Met-Cys for 30 min. Medium containing ³⁵S label was replaced with cold medium, and cell lysates were prepared after either a 40- or a 90-min chase. nsP1a-specific products were immunoprecipitated with pAb 5-6 and analyzed as described in the legend to Fig. 1A.