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. 2002 Feb;76(4):1707–1717. doi: 10.1128/JVI.76.4.1707-1717.2002

FIG. 7.

FIG. 7.

Comparison of promoter recognition by the recombinant p88 and p88C and the plant TCV RdRps. (A) Schematic representation of constructs tested in in vitro RdRp assays. The actual 3"-end sequence of each construct is shown in 3"→5" orientation. The core plus-strand initiation promoter for satC(−) (13) is shown in a black box (construct cTCV). Constructs cPR21 and cPR11 contain the extended and the core plus-strand initiation promoter for minus-strand DI-72 of TBSV, respectively (Panavas et al., submitted). The core minus-strand synthesis promoter for DI-72 of TBSV is boxed (construct gPR). The artificial AU-rich and GC-rich promoter sequences are shown in gray boxes (constructs A/U and G/C). The sites of expected initiation products are shown with arrows. In addition to the sequences shown, each construct contains the same 5" sequences derived from minus-strand MDV (221 nt) and a 17-nt anchor (Anc) sequence from TBSV (positions 4666 to 4682). (B to D) Representative denaturing gel analyses of radiolabeled RNA products synthesized by in vitro transcription with p88, p88C, and the TCV plant RdRp. Template-sized products are labeled with asterisks. The order of samples is the same in each panel.