FIG. 1.

(A) Schematic diagram of Sabin 1-derived RPS-Vax viral genome and RPS-Vax-derived recombinant PVs. The RPS-Vax genome contains a multiple cloning site (MCS) and 3C protease cutting site at the N-terminal end of the long polyprotein. A foreign insert integrated into the MCS can easily be detected by RT-PCR with the primer set indicated by arrows. (B) Schematic illustration of the template- and ligation-free PCR procedures that was used for the synthesis of long heteromultimeric concatemers or heavily sequence-adjusted inserts without template DNA. CR, complementary region. The solid triangles represent the mutation sites on the synthetic DNA. The circled numbers 1 and 8 represent long synthetic primers of between 60 and 100 bases in length.