FIG. 7.

Genetic stability of recombinant PVs in vivo. PVR-transgenic mice were inoculated intracerebrally with recombinant PV containing the original (PVm-150) and the sequence-adjusted (PVm-150/M) heteromultimeric insert. Every day, viruses were recovered from the spleen of each inoculated mouse and followed by a single round of amplification in HeLa cells. Viruses recovered from the passages in vivo were examined for their genetic integrity by RT-PCR. C denotes the insert-containing recombinant plasmid. The number indicates the day after infection on which the recombinant PV was recovered. The bar and arrow indicate the intact and the truncated forms of the inserts, respectively, which were generated during the replication of recombinant PV in vivo.