FIG. 2.

Complementation of the E1A-E1B cell lines for Ad-GFP vector production. Plasmid pST-E1AB or pST-E1AB-R was transfected into HeLa-tet-off cells together with a puromycin selection marker plasmid. Puromycin-resistant clones were screened for their ability to complement Ad-GFP production. The representative clones shown here were infected with Ad-GFP (MOI = 5) in the presence or absence of DOX and incubated for 72 h. The yields of Ad-GFP vector were determined by titer determination on 293 cells.