FIG.3.

Reactivation of the TET-inducible TRE promoter by TSA after promoter shutoff. (A) Ad-GFP production before and after reactivation of the TRE promoter in E1-112 cells by TSA. The E1-112 cells were infected with Ad-GFP (MOI = 5) in the absence of DOX and without or with TSA treatment (see Materials and Methods). Shown here are the Ad-GFP vectors produced by the E1-112 cells and with titers determined on 293 cells. The Ad-GFP yields were 2.9 × 10⁶ t.u./10-cm-diameter plate without induction and 1.8 × 10⁹ t.u./10-cm-diameter plate with induction. (B) Effects of TSA concentrations on Ad-GFP production in the E1-112 cell line. (C) Effects of TSA incubation time on Ad-GFP production in the E1-112 cell line.