FIG. 4.

Western analysis of E1A gene expression after TSA and DOX induction. (A) E1A gene expression in three E1 cell lines was examined to see if the gene shutoff can be reversed by TSA. The cells were treated with TSA for 12 h, and E1A gene expression was measured throughout the time course from the beginning of TSA treatment in the absence of DOX repression. 293 cells were used as the positive control for E1A expression without any treatment. (B) E1A gene reactivation and induction in the E1-112 cell line is dependent on both TSA treatment and removal of DOX repression.