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Western analysis of capsid gene expression from different cell lines. Various AAV-GFP cell lines were infected with Ad-LacZ in the absence of DOX. The E1A gene was induced by treating the cells with TSA for 12 h. The cell line XX-53-GFP is an AAV packaging cell line lacking the E1 genes. Only wt Ad infection can lead to AAV-GFP production in this cell line. Western analysis was performed with an anticapsid polyclonal antibody which recognizes all three capsid proteins.
