TABLE 1.
Ad-GFP vector yields in E1A-E1B HeLa-tet-off cells after serial passages
| Cell line | Passage | Ad-GFP (t.u./10-cm-diameter plate)a |
|---|---|---|
| E1-41-R | 1 | 2.6 × 109 |
| 3 | 5.0 × 108 | |
| 6 | 1.6 × 107 | |
| E1-39-R | 1 | 5.6 × 108 |
| 3 | 2.0 × 108 | |
| 6 | 3.7 × 107 | |
| E1-112 | 1 | 1.0 × 109 |
| 3 | 4.2 × 108 | |
| 8 | 1.9 × 107 |
a
The Ad-GFP titers are mean values from three experiments performed with 10-cm-diameter plates of the indicated cell lines. The tranducing units were determined by infecting HeLa cells with various dilutions of Ad-GFP virus stock. Under the fluorescence microscope, each green cell was translated into 1 t.u.