TABLE 2.

Yields of AAV-GFP vector from different AAV-GFP packaging cell lines after infection with helper Ad with E1A, E1B, and E3 deleted

Cell line	AAV vector yield (t.u./10-cm-diameter plate)a
	Initial	Optimized
GFP-60	1.2 × 106	NDb
GFP-113	3.3 × 106	ND
GFP-108	7.0 × 106	ND
GFP-174	1.2 × 107	6.3 × 107
GFP-214	1.18 × 107	2.0 × 108
GFP-265	6.6 × 105	ND

^aThe AAV-GFP yields are mean values from two experiments. The tranducing units were determined by infecting HeLa cells with various dilutions of AAV-GFP virus stock. Each GFP-positive cell was translated into 1 t.u.

^bND, not determined.