FIG. 7.

Production of replicative-form DNA (dsRF DNA) and genomic ssDNA after transfection of cells with the wild-type (CPV-wt) or mutant (Table 1) infectious plasmids. The DNA recovered by a modified Hirt lysis procedure was digested with DpnI prior to electrophoresis in a 1% agarose gel with 1 μg of ethidium bromide per ml. The viral DNAs were detected by Southern blotting.