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TABLE 1.

Detection of PPV in upper noninoculated leaves of transgenic tobacco plants at different days postinoculation

Transgenic line 15 d.p.i.
30 d.p.i.
45 d.p.i.
60 d.p.i.
No. of plants with PPV/no. of inoculated plantsa Position of highest leaf with virusb Plant sizec No. of plants with PPV/no. of inoculated plants Position of highest leaf with virus Plant size No. of plants with PPV/no. of inoculated plants Position of highest leaf with virus Plant size No. of plants with PPV/no. of inoculated plants Position of highest leaf with virus Plant size
U6B 3-4 2/8 5 8 4/8 7 12 1/8 7 16
1031-1XB 6/8 6 10-11 7/8 6 14-15 24/26 36
1031-2XC 7/8 6 5-6 8/8 9 9-12 6/8 11 16/17 6/8 14 20-26
1031-3XE 8/8 8 8-10 8/8 12-13 14-15 8/8 15 24/26 7/8 20 34
1210-6XI 6 11 7/8 NDd ND 2/8 ND ND
1115-1XK-1 10-11 16-20 22/31 39
1115-3XK-2 10-11 17-19 26/33 39
Vector trans. 7-8 17-20 28/30 28-30
Xanthi nc 7-8 12-14 25/29 35
a

As shown by ELISA.

b

Position above the three inoculated ones of the highest leaf showing PPV.

c

Total number of leaves above the three inoculated ones.

d

ND, not determined; upper leaves budded as bunches, preventing a confident assessment of leaf positions.