FIG. 5.

Three-dimensional model of the JE virus envelope glycoprotein ectodomain showing locations of mutations in the hinge region occurring with adaptation in FRhL or Vero cells (see Fig. 3). The sequence of the JE virus envelope glycoprotein (strain JaOArS982) (32) was aligned to one of the TBE virus structural template (31) as an input for automated homology modeling building by the method of SegMod (segment match modeling) using LOOK software (Molecular Application Group, Palo Alto, Calif.). The three domains are colored red (domain I), yellow (domain II), and blue (domain III).