TABLE 4.
Viremia in rhesus monkeys inoculated i.c. with YF-VAX or ChimeriVax JE FRhL3 and FRhL5 virusesa
| Test virus | Animal | Serum virus titer (log10 PFU/mL) on day:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | ||
| YF-VAX control | RT702M | —b | — | 1.6 | 3.0 | — | — | — | — | — |
| RAH80F | — | — | — | 3.3 | 2.5 | — | — | — | — | |
| RT758M | — | — | 2.1 | 3.2 | 2.8 | — | — | — | — | |
| RAL02F | — | — | — | 1.3 | — | — | — | — | — | |
| RT653M | — | — | — | 2.7 | — | — | — | — | — | |
| RT698F | — | 1.0 | 2.3 | 3.7 | 2.5 | — | 1.0 | — | — | |
| RT776M | — | — | — | — | — | — | — | — | — | |
| RAI12F | — | — | — | 2.0 | 2.5 | 2.5 | 2.0 | — | — | |
| RT621M | — | 1.0 | 2.0 | 3.3 | 2.0 | — | — | — | — | |
| c> | ||||||||||
| RP942F | — | 1.0 | 2.6 | 3.6 | 2.0 | — | — | — | — | |
| Meanc | 0.8 | 1.4 | 2.7 | 1.7 | 0.9 | 0.9 | ||||
| SD | 0.1 | 0.8 | 1.0 | 0.9 | 0.6 | 0.4 | ||||
| ChimeriVax-JE FRhL3, E279 Met | RAJ98F | — | — | 1.9 | 1.3 | — | — | — | — | — |
| RT452M | — | 1.3 | 2.1 | 1.6 | — | — | — | — | — | |
| RAR08F | — | — | 1.3 | 2.2 | 2.2 | 1.8 | — | — | — | |
| RR257M | — | — | 1.9 | 2.2 | 1.8 | — | — | — | — | |
| RV481F | — | — | 2.1 | 1.8 | 1.5 | — | — | — | — | |
| RT834M | — | — | 2.5 | 1.3 | — | — | — | — | — | |
| RT841F | — | — | 2.4 | 1.7 | — | — | — | — | — | |
| RT620M | — | — | 1.6 | 1.0 | — | — | — | — | — | |
| RT392F | — | — | — | — | — | — | — | — | — | |
| RT288M | — | — | — | — | — | — | — | — | — | |
| Mean | 0.8 | 1.7 | 1.5 | 1.0 | 0.8 | |||||
| SD | 0.2 | 0.6 | 0.5 | 0.6 | 0.3 | |||||
| P valued | 0.696 | 0.386 | 0.003 | 0.065 | 0.745 | |||||
| ChimeriVax-JE FRhL5 E279 Lys | RT628M | — | — | — | — | — | — | — | — | — |
| RAJ55F | — | — | — | — | — | — | — | — | — | |
| RT678M | — | — | — | — | — | — | — | — | — | |
| RT769F | — | — | — | 2.0 | — | — | — | — | — | |
| RT581M | — | — | — | — | — | — | — | — | — | |
| RAK22F | — | — | — | — | — | — | 1.8 | — | — | |
| RR726M | — | — | — | — | — | — | — | — | — | |
| RT207F | — | — | — | — | — | — | — | — | — | |
| RR725M | — | — | — | — | — | — | — | — | — | |
| RT490F | — | — | — | — | — | — | — | — | — | |
| Mean | 0.7 | 0.7 | 0.8 | 0.7 | 0.7 | 0.8 | ||||
| SD | 0.0 | 0.0 | 0.4 | 0.0 | 0.0 | 0.4 | ||||
| P valuee | 0.331 | <0.000 | 0.010 | 0.076 | 1.0 | 1.0 | ||||
a
For the dose inoculated, see Table 3. In most tests undiluted serum was tested, with the cutoff being 1.0 log10 PFU/ml, in some cases, undiluted serum was toxic to cells, and serum diluted 1:2 or 1:5 was used (cutoff, 1.3 or 1.7 log10 PFU/ml).
b
—, no detectable viremia.
c
For the purpose of calculating mean titers and standard deviations, 0.7 was used in place of<1.0, 1.0 was used in place of <1.3, and 1.4 was used in place of <1.7.
d
Comparison with YF-VAX by two-tailed t test.
e
Comparison with ChimeriVax-JE FRhL3 by two-tailed t test.