FIG. 5.

Major by-product obtained in the recovery of integrated viral DNA. (A) Schematic drawing of the generation of the plasmid. M, MluI; B, BamHI; C, ClaI; C*, ClaI site within gag gene subjected to dam methylation in E. coli but not in avian cells. (B) Restriction enzyme analyses of the plasmid. Digestion with ClaI did not cut the plasmid due to dam methylation. Digestion with MluI cut the plasmid once and digestion with BamHI generated two fragments, of 1.54 and 1.39 kb, as expected. kb, DNA ladder in kilobases.