. 2002 Feb;76(4):1642–1648. doi: 10.1128/JVI.76.4.1642-1648.2002

TABLE 1.

Oligonucleotide primers for DNA amplification

Region	Specificity	Primer	Sequence (5" → 3")	Orientation	Positions	Location	Size (bp)^a	[Mg²⁺] (mM)^b
pX	Types 1 and 2	ATLpX1	CCCACTTCCCAGGGTTTGGACAGAGTCTT	Sense	7432-7461^c
		ATLpX5	GGAGGGGAGTCGAGGGATAAGG	Antisense	7637-7658^c	Outer	226	6
		SK43	CGGATACCCAGTCTACGTGT	Sense	7466-7485^c
		SK44	GAGCCGATAACGCGTCCATCG	Antisense	7604-7624^c	Inner	158	6
	Type L	AV45L^d	GGACGCGTTGTCAGCTC	Sense	7432-7448^e
		AV46L^d	GGGGGAGAGCTGGTAGAGGTA	Antisense	7714-7734^e	Outer	302	4
		AV42L^d	CTCCCCTCCTTCCCCAC	Sense	7474-7490^e
		AV43L^d	CCACATGGTGTATACGTTTTGG	Antisense	7671-7692^e	Inner	218	4
		AV51L^d	ACAATTGCCTCGAGCTCACCC	Sense	7601-7621^e
		AV82L^d	GAGGCACACGACGGA	Antisense	7696-7713^e	Inner	112	6
LTR	Type 1	ATLTR1	TGACACTGACCATGAGCCCCAAAT	Sense	130-153^c
		ATLTR2	TCGTATCCCGGACGAGCCCCCAA	Antisense	886-908^c	Outer	778	6
		ATLTR11	ACTAAGGCTCTGACGTCTCCCCC	Sense	228-250^c
		ATLTR12	CGGTACTTGGCCGTGGGCCAAGCCG	Antisense	790-814^c	Inner	586	6
	Type L	LTR-1L	CAACAACCACCAACTAGGGG	Sense	8260-8279^e
		LTR-2L	AATGTTACAGGCGCTGG	Antisense	8841-8857^e	Outer	597	3
		LTR-3L	CAAATAGCTGAATCATCCGTCTG	Sense	8281-8303^e
		LTR-4L	GCAACAGAAGTGCTACTTTCG	Antisense	8797-8817^e	Inner	536	3

Size indicates the expected fragment size.

[Mg²⁺], MgCl₂ concentrations for PCR.

Nucleotide positions correspond to the full genome sequence of ATK, the prototype strain of HTLV-1 (J02029).

These primers, originally developed as a generic PCR system for PTLV, were modified to specifically amplify STLV-L.

Nucleotide positions corresponds to the full genome sequence of PH969, the prototype strain of PTLV-L (Z29673).