FIG. 2.

Immunofluorescence analysis of DC subset distribution in human Peyer's patches. Human terminal ileum tissues were embedded in OCT medium, and 6- to 8-μm frozen sections were stained with antibodies against MHC class II (HLA-DR/DP/DQ) (green) and CD11c (red) (A and B) or IL-3R (CD123; red) (C and D). The subepithelial dome region (B) and the interfollicular regions (D) containing CD11c⁺/MHC class II⁺ or IL-3R⁺/MHC class II⁺ DCs, respectively, are shown at higher magnifications. Images were captured using either 10× (A and C) or 40× (B and D) objective lenses. No background staining was detected when mouse IgG was used as a control (data not shown). The nucleus was stained with DAPI (blue). White bar, 100 μm in this and all subsequent figures. This figure gives results representative of three different donor tissues.