FIG. 9.

Blocking of rhesus macaque DC-SIGN-mediated SIV transmission by anti-DC-SIGN antibodies. T-Rex cells that express rhesus macaque DC-SIGN under a doxycycline-inducible promoter and control T-Rex cells were seeded in 96-well plates and induced with doxycycline. The cells were incubated with 20 μg of the indicated antibodies or mannan per ml and then pulsed with SIVmac239 MER Env replication-competent luciferase reporter virus. After a 3-h incubation, the cells were vigorously washed and cocultivated with CEMx174 target cells. After 72 h the cocultures were lysed and the luciferase activity was quantified. The results are presented as percent transmission ± standard deviation observed from untreated cells. A representative experiment carried out in triplicate is shown, and similar results were obtained in two independent experiments.