FIG. 2.

Assembly and egress of ILTV and mutant PrV. Intranuclear assembly (A), formation of perinuclear virions (B and C), and fusion with the outer nuclear membrane (D) do not differ morphologically from those of the other alphaherpesviruses examined (34). However, large amounts of tegument material may be incorporated into virions (G and H), which is clearly observed to occur in the cytoplasm only (E to F; see also reference 34). (G) An enveloped ILTV virion inside a vesicle. (H) An extracellular released virion. In panel E on the right, a capsidless L particle is demonstrated. (I) Intracytoplasmic PrV capsid accumulations without apparent tegument in the absence of the UL37 protein (45). (J) Accumulations of tegumented PrV capsids in the absence of gE/gI and gM (6). Bars, 150 nm in panels A to H and 250 nm in panels I and J.