TABLE 2.
Comparison of the three virus producer cell lines used for insertional mutagenesis
Virus/producer cell line | % Good founders | % Survival of injected embryos at 24 h postinjection | No. of founders/day/person | EAV avg | % with EAV ≥ 5 | No. of insertions/founder | Person-days needed to make 106 GLa insertions |
---|---|---|---|---|---|---|---|
SFG/A7 | 15 | 45 | 60 | NAc | NA | 12 | >1,390 |
SFG/F5 | 75 | 37 | 50 | 3.6 | 25 | 18b | 1,110 |
GT/186 | 100 | 57 | 200 | 14.3 | 96 | 27b | 185 |
GL, germ line.
Estimate.
NA, not assayed.