FIG. 3.

The Vpr-CypA chimera rescues the infectivity of CypA-deficient viruses. 293T cells were transfected with proviral DNA encoding wild-type R9 or R9 G89V CA mutant and were cotransfected with Vpr-CypA or Vpr-Pr-CypA in the presence □ or the absence ▪ of CsA. Viruses (1 ng of p24) were tested for their capacity to infect CD4⁺ HeLa cells. Infectivity was monitored by X-Gal staining. Results (triplicates) are expressed as number of blue cells per nanogram of p24. Results are representative of two independent experiments.