Skip to main content
. 2002 Mar;76(5):2255–2262. doi: 10.1128/jvi.76.5.2255-2262.2002

FIG. 4.

FIG. 4.

In-trans incorporation of CypA mutant which lacks isomerase activity. 293T cells were transfected with a proviral DNA encoding wild-type R9 (lanes 1 to 12) or cotransfected with a construct encoding Vpr-Pr alone (lanes 3, 4, 9, and 10), Vpr-Pr-CypA (lanes 5 and 6), or Vpr-Pr-CypA H126A (lanes 11 and 12) in the presence (lanes 2, 4, 6, 8, 10, and 12) or absence (lanes 1, 3, 5, 7, 9, and 11) of 20 μM CsA. Produced viruses were analyzed for CypA, CA, and Vpr content by immunoblotting using specific antibodies. −, control.