Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Mar;76(5):2255–2262. doi: 10.1128/jvi.76.5.2255-2262.2002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, American Society for Microbiology

PMC Copyright notice

FIG.5. — The isomerase activity of CypA is dispensable for HIV-1 infectivity. (A) 293T cells were transfected with proviral DNA encoding wild-type R9 or R9 G89V CA mutant and were cotransfected with Vpr-Pr, Vpr-Pr-CypA, Vpr-Pr-CypA H126A, or Vpr-Pr-CypA Δ4 (RKKK148/151/154/155AAAA) in the presence (□) or the absence (▪) of CsA. Viruses (1 ng of p24) were tested for their capacity to infect CD4⁺ HeLa cells. Infectivity was monitored by X-Gal staining. Results (duplicates) are expressed in number of blue cells per nanogram of p24. Results are representative of two independent experiments. (B) 293T cells were transfected with proviral DNA (20 μg) encoding R9 G89V CA mutant and were cotransfected with increasing concentrations of Vpr-Pr-CypA H126A (1, 5, 10, and 20 μg of DNA; lanes 1, 2, 3, and 4, respectively) in the presence of CsA. After p24 standardization, produced viruses were analyzed for CypA content by immunoblotting using anti-CypA antibodies and for infectivity using CD4⁺ HeLa cells. Infectivity was monitored by X-Gal staining. Results (duplicates) are expressed in number of blue cells per nanogram of p24. Results are representative of two independent experiments.

FIG.5. — The isomerase activity of CypA is dispensable for HIV-1 infectivity. (A) 293T cells were transfected with proviral DNA encoding wild-type R9 or R9 G89V CA mutant and were cotransfected with Vpr-Pr, Vpr-Pr-CypA, Vpr-Pr-CypA H126A, or Vpr-Pr-CypA Δ4 (RKKK148/151/154/155AAAA) in the presence (□) or the absence (▪) of CsA. Viruses (1 ng of p24) were tested for their capacity to infect CD4⁺ HeLa cells. Infectivity was monitored by X-Gal staining. Results (duplicates) are expressed in number of blue cells per nanogram of p24. Results are representative of two independent experiments. (B) 293T cells were transfected with proviral DNA (20 μg) encoding R9 G89V CA mutant and were cotransfected with increasing concentrations of Vpr-Pr-CypA H126A (1, 5, 10, and 20 μg of DNA; lanes 1, 2, 3, and 4, respectively) in the presence of CsA. After p24 standardization, produced viruses were analyzed for CypA content by immunoblotting using anti-CypA antibodies and for infectivity using CD4⁺ HeLa cells. Infectivity was monitored by X-Gal staining. Results (duplicates) are expressed in number of blue cells per nanogram of p24. Results are representative of two independent experiments.