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Infectious titer of SIVmac239ΔV1V2. Viral stocks were normalized for p27 content and then used for duplicate infections of CEMx174SIV-SEAP indicator cells, which contain a Tat-inducible gene for SEAP. SEAP activity was measured 72 h postinfection, a measure which principally reflects the first round of infection (26). Indicator cells were the same cells as those used in the neutralization assay (see Materials and Methods). Error bars show standard deviations.
