TABLE.
3. Thymocyte subpopulation distribution in weeks 1 to 8 following inoculation of neonatal NIH/Swiss mice with SL3 or SL3ΔMyb5a
| Subpopulation | Wk p.i. | % (mean ± SEM)b in:
|
||
|---|---|---|---|---|
| SL3-inoculated mice | SL3ΔMyb5-inoculated mice | Uninfected controls | ||
| CD4− CD8− (DN) | 1 | 15.8 ± 2.1 | 7.7∗ ± 3.7 | 16.3 ± 2.5 |
| 2 | 11.6 ± 1.9 | 10.3 ± 1.8 | 10.5 ± 1.8 | |
| 3 | 19.0 ± 5.1 | 12.6 ± 1.9 | 17.9 ± 3.1 | |
| 4 | 19.0∗ ± 2.9 | 12.2 ± 3.6 | 5.4 ± 0.6 | |
| 5 | 19.0∗ ± 3.3 | 15.5 ± 3.5 | 10.7 ± 1.7 | |
| 6 | 22.6 ± 4.2 | 27.2∗ ± 6.3 | 16.4 ± 0.7 | |
| 7 | 19.2∗ ± 1.0 | 15.5 ± 3.9 | 13.8 ± 1.9 | |
| 8 | 22.0 ± 4.1 | 16.5 ± 3.4 | 23.1 ± 1.8 | |
| CD4+ CD8+ (DP) | 1 | 65.2 ± 4.5 | 70.3 ± 4.4 | 70.0 ± 2.1 |
| 2 | 65.3 ± 6.1 | 75.7 ± 2.4 | 73.4 ± 0.9 | |
| 3 | 59.5 ± 5.1 | 64.5 ± 6.5 | 58.2 ± 3.2 | |
| 4 | 58.3∗ ± 2.1 | 67.1∗ ± 3.9 | 77.6 ± 2.6 | |
| 5 | 61.9 ± 3.8 | 64.2 ± 2.2 | 70.1 ± 2.8 | |
| 6 | 55.6∗ ± 3.5 | 50.1∗ ± 4.3 | 63.5 ± 3.0 | |
| 7 | 48.9∗ ± 1.9 | 62.6 ± 3.7 | 58.4 ± 4.4 | |
| 8 | 57.3 ± 4.8 | 56.2 ± 2.6 | 48.7 ± 4.7 | |
| CD4+ CD8− (CD4) | 1 | 17.3 ± 5.7 | 16.7 ± 5.7 | 11.1 ± 2.5 |
| 2 | 20.1 ± 4.7 | 11.6 ± 1.4 | 13.9 ± 2.1 | |
| 3 | 17.5 ± 2.5 | 19.0 ± 4.9 | 19.7 ± 2.1 | |
| 4 | 21.5∗ ± 0.8 | 18.1 ± 1.0 | 15.0 ± 2.3 | |
| 5 | 14.6 ± 1.9 | 16.7 ± 2.6 | 13.6 ± 3.2 | |
| 6 | 16.9 ± 2.0 | 18.1 ± 3.4 | 16.5 ± 2.9 | |
| 7 | 23.7 ± 1.4 | 18.6 ± 1.3 | 21.2 ± 3.0 | |
| 8 | 14.7 ± 5.0 | 19.6 ± 2.5 | 22.5 ± 1.8 | |
| CD4− CD8+ (CD8) | 1 | 1.6 ± 0.4 | 2.6 ± 1.7 | 2.5 ± 1.2 |
| 2 | 2.0 ± 0.6 | 2.1 ± 0.7 | 1.9 ± 0.2 | |
| 3 | 3.9 ± 0.4 | 3.7 ± 0.4 | 4.3 ± 0.7 | |
| 4 | 1.5 ± 0.3 | 2.6 ± 0.5 | 2.1 ± 0.4 | |
| 5 | 4.6 ± 1.2 | 3.7* ± 0.3 | 5.6 ± 0.7 | |
| 6 | 5.0 ± 0.9 | 3.9 ± 0.5 | 3.6 ± 0.9 | |
| 7 | 8.2 ± 1.0 | 3.3 ± 1.2 | 6.6 ± 1.6 | |
| 8 | 6.0 ± 2.8 | 5.4 ± 1.9 | 5.7 ± 1.3 | |
Relative proportions of the thymocyte subpopulations CD4− CD8− (double negative [DN]), CD4+ CD8+ (double positive [DP]), CD4− CD8+ (single positive [CD8]) and CD4+ CD8− (single positive [CD4]) were examined at weekly intervals following inoculation of neonatal NIH/Swiss mice with SL3 MuLV or SL3ΔMyb5. At each timed collection, single-cell thymocyte suspensions were prepared and dually stained with FITC-conjugated anti-CD4 and PE-conjugated anti-CD8. The percentage of thymocytes staining for one or both markers was assessed by flow cytometry and compared to values from age-matched uninfected control animals examined in parallel. Four to seven animals were examined at each timed collection.
Asterisks indicate values from SL3- or SL3ΔMyb5-infected mice that are statistically distinct from those from age-matched uninfected controls as determined by Student's t test (P < 0.05).