FIG. 7.

Transcriptional activation assays. Lysates were prepared from C33A cells cotransfected with a CAT reporter plasmid in which the CAT gene is under the control of the EBV FR element and with pc3oriP plasmids expressing EBNA1 (solid squares), Δ8-67 (solid circles), Δ8-67/Δ325-376 (solid triangles), Δ8-67/Δ330-354 (open squares), Δ34-52 (solid diamonds), Δ61-83 (open triangles), Δ325-376 (open diamonds), or no EBNA1 (pc3oriP; open circles). Equal amounts of lysates were tested for chloramphenicol acetylation rates as a measure of CAT expression levels. Percentages of chloramphenicol acetylated after various reaction times are shown.