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Immunoblot analysis of NCp9. Ten micrograms of whole-cell extract from yTM443 cells overexpressing WT or IN-mutant Ty3 was separated on an SDS-10% polyacrylamide gel and subjected to immunoblot analysis with polyclonal rabbit α-NCp9 IgG. Secondary antibodies to rabbit IgG were detected by chemiluminescence. The positions of Ty3 Gag3p-derived proteins p38 and NCp9 are indicated by arrows on the left. Labels are as for Fig. 1.
