FIG. 5.
Northern analysis of VLP tRNAiMet. VLP RNA was isolated from fractions of VLPs from cells expressing wild-type (WT) or IN-mutant Ty3, containing 20 μg of protein or equivalent fractions from nontransformed cells (NTC). The RNA was separated on a denaturing 8% polyacrylamide gel next to 3 ng of size marker tRNAiMet produced by in vitro transcription (IVT tRNAiMet). The RNA was subjected to Northern analysis with a tRNAiMet-specific, 32P-5′-end-labeled oligonucleotide probe. tRNAiMet is indicated by the arrow on the left. The panels indicate results from two separate blots. Labels are as described in the legend to Fig. 1.