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. 2002 Mar;76(6):2835–2847. doi: 10.1128/JVI.76.6.2835-2847.2002

FIG. 5.

FIG. 5.

Carbohydrate analysis of purified o-gp140. (A) Immunoblot detection of biotinylated gp120 US4 (lane 1) and o-gp140 US4 (lane 2) using streptavidin-HRP. (B) Carbohydrate linkage analysis of o-gp140 using different enzymes. o-gp140 was digested with NANase (lane 2), O-glycosidase (lane 3), and PNGF (lane 4) separately and together (lane 5). o-gp140 without any enzyme was used as control (lane 1), and molecular mass standards (in kilodaltons) are also indicated. (C) endo-H digestion of gp120 US4 (lanes 2 and 3) and o-gp140 US4 (lanes 4 and 5). + and −, proteins with and without endo-H, respectively.