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Intracellular localization of MV-LAP. RK13 cells grown on coverslips were transfected with expression vectors for GFP-tagged versions of MV-LAP. Cells were fixed 36 h posttransfection, and nuclei were stained with propidium iodide (red in panels A and D; yellow upon merging with GFP as in panels B and C). (A) Full-length MV-LAP-GFP fusion; (B) GFP control; (C) N-term-LAP-GFP; (D) C-term-LAP-GFP.
