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. 2002 Mar;76(6):2936–2951. doi: 10.1128/JVI.76.6.2936-2951.2002

FIG. 2.

FIG. 2.

DC-captured AT-2 SIV retains its fusogenic capacity. (A) Immature and mature human DCs were exposed to 30 ng of AT-2 SIV p27 Ag versus heat-treated AT-2 SIV per 105 cells (2 h at 37°C), washed, and cocultured with untreated CEMx174 cells at a DC-to-CEMx174 cell ratio of 1:10. After 2 h at 37°C, cytospins were prepared and the numbers of syncytia per cytospin were counted. The mean numbers of syncytia (± standard deviation) per slide from six independent experiments are shown. (B) DCs were incubated in the presence of AT-2 SIV at 30, 3, or 0.3 ng of p27 Ag/105 cells and washed, and 104 virus-loaded DCs were cocultured with 105 CEMx174 cells for 2 h at 37°C (CEMx174 + DC-AT-2). Alongside, cell-free AT-2 SIV was added at 30, 3, or 0.3 ng of p27 Ag per 105 CEMx174 cells (CEMx174 + cell-free AT-2). The numbers of syncytia were determined as described for panel A. Results of one representative experiment out of two are shown.