TABLE 2.
HIV-1 p24 levels in iDC and mDC loaded with untreated and PUV-treated, HIV-1 IIIB-infected CD8− autologous cells
| Cell cultureb | Median HIV-1 p24 level in ng/ml (range)a at:
|
||
|---|---|---|---|
| 0 h | 36 h | 60 h | |
| CD8− IIIB | 0.12 (0.09-0.48) | 1.55 (0.72-11.1) | 2.07 (1.06-14.0) |
| PUV CD8− IIIB | 0.18 (0.17-0.56) | 0.74 (0.73-3.35) | 0.45 (0.29-2.08) |
| CD8− IIIB + iDC | 0.10 (0.10-0.45) | 29.6 (1.07->53.6) | 25.0 (6.8->53.6) |
| PUV CD8− IIIB + iDC | 0.19 (0.04-0.19) | 0.69 (0.11-0.80) | 0.44 (0.18-0.46) |
| CD8− IIIB + mDC | 0.10 (0.10-0.45) | 27.2 (2.31->53.6) | 40.1 (12.1->53.6) |
| PUV CD8− IIIB + mDC | 0.19 (0.04-0.19) | 0.69 (0.03-0.75) | 0.51 (0.18-0.54) |
a
CD8− cells and DC were derived from PBMC of three normal, HIV-1-negative donors (N5, N6, and N7) and cultured for the designated times.
b
CD8− IIIB = HIV-1 IIIB-infected, autologous CD8− cells; PUV CD8− = PUV-treated, HIV-1 IIIB-infected, autologous CD8− cells.