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. Author manuscript; available in PMC: 2026 Sep 23.
Published in final edited form as: Mol Cell. 2026 Aug 6;86(16):3185–3197.e7. doi: 10.1016/j.molcel.2026.07.012

Figure 2: BRD4-S binds to nucleosomes with nanomolar affinity at 70 mM NaCl independent of histone acetylation.

Figure 2:

(a) Time-resolved FRET binding assay results for BRD4-S binding to unmodified (blue), H4 tailless = H4(24–102) (green) and H4 K12acK16ac nucleosomes (pink) in 70 mM NaCl. (b) Effect of NaCl concentration on BRD4-S binding to unmodified (blue) or H4 K12acK16ac nucleosomes (pink) as assayed by TR-FRET. P-values comparing the Kd for the acetylated vs unmodified nucleosomes indicated as follows: ns for not significant, * for p-value < 0.05, * for p-value < 0.01.