FIG. 2.

Characterization of HSV-induced RANTES secretion. (a and b) J774A.1 cells were seeded and infected with 3 × 10⁶ PFU of HSV-2 per ml (MOI of 6) or equivalent amounts of heat- or UV-inactivated virus. Supernatants were harvested at 24 h p.i. (a) or at the indicated time points (b) and assayed by ELISA. (c) Resting PCs were seeded and infected with 3 × 10⁶ PFU of HSV-1 or HSV-2 per ml (MOI of 1.7). Supernatants were harvested after 24 h of infection. RANTES protein levels in the supernatants were measured by ELISA. Results are shown as means from three or four cultures ± SEMs. For all panels, essentially similar results were obtained in three independent experiments.