FIG. 3.
Characterization of HSV-induced RANTES mRNA accumulation in J774A.1 cells. (a) The cells were infected with 3 × 106 PFU of HSV-2 per ml (MOI of 6) and total RNA was harvested at the indicated time points. (b) The cells were infected with 3 × 106 PFU of HSV-2 per ml (MOI of 6) or equivalent amounts of heat- or UV-inactivated virus. Twenty-four hours later, total RNA was harvested. The RNA was reverse transcribed with oligo(dT) priming and analyzed for the presence of RANTES and β-actin by PCR. (c) J774A.1 cells were mock infected or treated with infectious or UV-inactivated HSV-1 (MOIs of 6 and 2). After 3 h of infection, nuclear extracts were prepared and analyzed for the presence of VP16 by Western blotting. For all panels, similar results were obtained in three independent experiments.