FIG. 5.

Induction of RANTES production in the absence of PKR activity and specific viral proteins. RAW-pBK-CMV and RAW-PKR-M7 cells (a) or RAW 264.7 cells (b to e) were seeded and left overnight to settle. (a) The cells were mock infected or infected with 10⁶ PFU of HSV-1 or HSV-2 per ml (MOI of 2). (b to e) The cells were treated with mock virus preparation or infected with 10⁶ PFU of mutant virus (ICP0 mutant [b], ICP4 mutant [c], ICP22 mutant [d], or ICP27 mutant [e]) per ml (MOI or 2), wild-type HSV-1, or the reconstituted mutants. After 24 h of infection, supernatants were harvested and RANTES levels were measured by ELISA. Results are shown as means from three or four cultures ± SEMs. ND, not detectable. Similar results were seen in four independent experiments for panels a and b and two independent experiments for panels c to e.