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The cloned 1B3 (clone 1.4) virus exhibits the same replication kinetics in pt mPBMC as the uncloned 1B3 virus stock. The amount of input virus for the first pt mPBMC passage was normalized for p24. For infection of subsequent pt mPBMC passages, supernatants from the previous passage showing peak virus production were used. Days postinfection for each passage are labeled from the start of each infection of fresh pt mPBMC.
