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The inhibitory sequences in the HPV-16 L1 gene act in cis by reducing the mRNA levels in the nucleus. HeLa cells were transfected with plasmid pC16L1 or pC16L1MUT#123. The transfected cells were fractionated into nuclear and cytoplasmic fractions, and RNA was extracted and analyzed by Northern blotting to detect the L1 mRNAs (A) and 28S and 47S rRNAs (B), as described in Materials and Methods. N, nuclear RNA; C, cytoplasmic RNA.
