FIG. 2.

Expression of VHSV glycoprotein in rIHNV-Gvhsv-infected cells. EPC cells were infected with wild-type rIHNV or rIHNV-Gvhsv as indicated on the left. Cells were alcohol-acetone fixed and incubated first with a rabbit anti-IHNV polyclonal antiserum (αIHNV) and then with a mouse anti-VHSV G MAb (αG-VHSV). Expression was revealed with a mixture containing Texas red-conjugated goat anti-mouse immunoglobulin G (for αG-VHSV) and fluorescein isothiocyanate-conjugated goat anti-rabbit immunoglobulin G (for αIHNV). Overlapping of double labeling is shown in the right panels (Cross).