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. 2026 Jun 10;12:78. doi: 10.1038/s41526-026-00619-8

Table 3.

Study demographics

Study N (M/F) Age (yr) Body mass (kg) Category Description Suited condition Gravity condition Pace Actual intensity Task fail
Ade et al.29 70 (39 M/31 F) 23 ± 5 Not reported Task circuit

Material transport field test (all participants): 10x

(1) Material loading- loaded 10 × 10 kg simulated core samples into a 2-wheeled cart designed to simulate the modular equipment transporter used by the Apollo 14 astronauts

(2) Material transport- pushed cart across a 54 m level track

(3) Material unloading- removed the simulated core samples and returned them to a table

Device operations field test (24 participants): 5x

(1) Valve and bolt manipulation-turned 4 valves + bolts (5 cm) against a resistance which was provided by springs.

(2) Hose couplings- connected and disconnected 4 × 7.6 cm fire hose couplings

(3) Balance beam walk - traversed a 10 cm wide x 3.66 m long balance beam

(4) Equipment drag-pulled a weighted sled 18 m with a rope

Shirtsleeve (athletic apparel) 1 g As fast as possible Not reported

Material transport field test: 19/70 (27%)

Device operations field test: Not reported

Ade et al.30 21 (10 M/11 F) 26 ± 7 76.0 ± 15.7 Task circuit

Field test (10 m between each task):

(1) Ladder climb

(2) Agility cones

(3) Stair climb

(4) Lateral climb

(5) Equipment lift - moved 2 × 4.5 kg boxes from waist to eye level and 2 × 9 kg boxes from floor to waist level and back

(6) Step-entry lateral movement with 40 cm hurdles and ducked under chest-level poles

Shirtsleeve (athletic apparel) 1 g

Lunar: 20 mL/kg/min

Martian:

30 mL/kg/min

Lunar: ~19 mL/kg/min (~44% VO2pk)

Martian: ~28 mL/kg/min (~65% VO2pk)

Lunar: All completed

Martian: 6/21 (28%); could only complete 65% of the Martian test

Ryder et al.34 60 (32 M/28 F) 37 ± 7 (24–52) 75 ± 13.3 Task circuit

(1) Capsule egress: supine to stand, ladder deploy, lift and push the un-deployed life raft (13.6 kg) and 2 crew survival packs (6.4 kg each), climb out of the hatch

(2) Ambulation and supply transfer (1.5 km walk + 30 (10.9 kg) crew transfer bag carries 5 m)

(3) Rescue drag (mannikin (75 kg) rolled onto rescue sled, dragged 50 m)

(4) Hill climb and descent (2020 m (2–8% grade))

Five external load conditions in the weighted suit 1 g As fast as possible Not reported

Capsule egress: 2/290 trials

Ambulation/ transfer: 7/290 trials

Rescue drag: 2/290 trial

Hill climb: 28/283 trials

Ryder et al.33

20 (10 M/10 F)

6 (Shuttle Crew; 4 M/2 F)

36 ± 10 (25–54) 68.8 ± 14 Task circuit

(1) Seated egress and walk (once from upright, once from supine + obstacle course)

(2) Recovery from fall to stand (prone to standing)

(3) Hatch opening test (peak force turning wheel for 3–5 s; then turn wheel at 50% max torque for 20 s)

(4) Passive ladder climb test (40 rungs)

(5) Object translation (pick up and move tools, equipment, samples- 2.7–9 kg over 2.4 m)

Three external load conditions in the weighted suit per group 1 g As fast as possible Not reported Not reported
Taylor et al.36 8 (5 M/3 F) 34.9 ± 3.7 72.9 ± 8.3 Task circuit

(1) Hatch opening: Biodex upper extremity wheel attachment

(2) Hand drilling: Biodex lateral rotating pinch attachment

(3) Construction wrenching: Biodex upper extremity wrench attachment

(4) Inclined walk: 0.8 km on a treadmill (angle of inclination 1° every 2 min until it reached 5 degrees, decline at the same rate down to 0°)

(5) Sample collection: collect 9 medicine balls arranged in two successive rows at 3 and 6 m from a designated starting position; retrieve and deposit in a storage rack

(6) Emergency crewmember drag: drag a 54 kg dummy across 13.5 m (8.5 m straight, 90 degree turn, 5 m drag)

Equipped with weighted backpack plus distributed weights (48 kg) to simulate the NDX-2 space suit (ankle = 0.07 kg, chest 11.34 kg, waist = 9.07 kg, wrist = 2.29 kg, backpack = 15.88 kg) 1 g As fast as possible

Hatch opening: VO2: 24.8 ± 4.4; RPE: 6 9 ± 2.3

Hand drilling: VO2: 18.5 ± 3.0; RPE: 5.5 ± 2.1

Construction wrenching: VO2: 28.3 ± 6.3; RPE: 6.8 ± 1.9

Inclined walk: VO2: 32.3 ± 6.6; RPE: 6.3 ± 2.0

Sample collection: VO2: not reported; RPE: 4.3 ± 1.5

Emergency drag: VO2: not reported ; RPE: 4.8 ± 1.7

Not reported
Ade et al.28

71

(40 M/31 F)

23 ± 5 73.8 ± 15.7 Task circuit

Physical abilities field test (15 rounds):

(1) Ladder climb (12 ft ladder, 10 rungs)

(2) Agility cones, forward and backward movement through 6 vertical poles 6 ft apart

(3) Stair climb, 4.5 m set of stairs, each step 25 cm rise

(4) Lateral climb

(5) Box lift, 2 × 4.5 kg boxes from waist to eye level, then 2 × 9 kg boxes from floor to waist

(6) Step over task, moved laterally and stepped over 40 cm high hurdles and ducked under chest-level poles

Shirtsleeve (athletic apparel) 1 g As fast as possible Not reported Not reported
Standalone task 10 km Walkback
Alexander et al.31 15 (5 M/ 10 F) 47 ± 4 90 ± 23 Standalone task Capsule egress test: seated supine, roll over, move 2 × 5 kg bags 1 m, release and attach rope ladder to floor, carry 2 packages through top hatch, exit capsule through top hatch Shirtsleeve (athletic apparel) 1 g As fast as possible VO2 reached 72 ± 25% of relative VO2pk No failures
Norcross et al.39 6 (6 M)

46.8 ± 4.3

(40–51)

81.4 ± 7.8 Standalone tasks Treadmill ambulation (POGO) POGO: shirtsleeve with PLSS backpack mock-up [18 kg] + gimbal support system [41 kg] 1/6 g Self-paced walking and running

Unsuited: 8.8–16.6 mL/kg/min

Suited: 16.7-29.3 mL/kg/min

Not reported
3/8 g

Unsuited: 11–12.1 mL/kg/min

Suited: 27.6-35.7 mL/kg/min

10 km walkback (0% grade treadmill) Mark III suit (MKIII) 1/6 g Self-paced walking and running

Avg %VO2pk: 50.8 ± 6.1% (45–61%)

Avg speed: 1.74 ± 0.22 m/s (6.3 km/h)

Avg VO2: 2.0 ± 0.3 L/min

Total energy expenditure: 944.2 ± 70.5 kcal

RPE: 11.8 ± 1.6

All completed within 2 h
Sutterfield et al.35 45 (20 M/25 F) 39 ± 10 79 ± 20 Standalone tasks

(1) 1500 m treadmill traverse (constant speed 0.85 m/s; on track with synthetic rocks and pebbles to simulate Mars surface) followed by 30 × 10 lb bag carries for 10 m each

(2) 2000 m up/down Hill climb (0–8% grade; 55–99 m/min or 3.3–5.9 km/h) carrying 5.5 kg body bar on ascent, no bar on descent

Shirtsleeve (athletic apparel) 1 g Low intensity: 17–20 mL/kg/min

Hill climb: 20 ± 2.3 mL/kg/min (~56% VO2pk)

Surface Traverse: 24.0 ± 1.8 mL/kg/min (~67% of VO2pk)

Hill climb: 6/45 (13%)

Surface Traverse: 6/45 (13%)

Moderate intensity: Pacing + 13.6 kg (torso) + 2.3 kg (each ankle + upper arm)

Hill Climb: 26.0 ± 0.7 mL/kg/min ( ~ 73% VO2pk)

Surface traverse: 28.7 ± 1.8 mL/kg/min ( ~ 80% VO2pk)

Hill climb: 10/39 (25%)

Surface traverse: 15/39 (38.5%)

Mulavara et al.38

13 (ISS crew; 11 M/2 F)

10 (BR no ex; 10 M)

9 (BR ex; 9 M)

47 ± 5

38 ± 7

34 ± 6

84 ± 14

80 ± 9

77 ± 7

Standalone tasks

(1) Seated egress and walk

(2) Recovery from fall to stand

(3) Object translation (pick up and move tools, equipment, samples- 2.7, 4.5, 9 kg 2.4 m)

(4) Jump down test (30 cm)

(5) passive ladder climb test (40 rungs)

(6) Activity board: moving 2 hose connectors from their initial attachments to an adjacent attachment, moving 3 electrical-connectors from vertical positions to horizontal positions, and removing and installing a handle using a cordless power driver to loosen 2 screw

(7) Hatch opening test (peak force turning wheel for 3–5 s)

Shirtsleeve (athletic apparel) 1 g Self-paced Not reported Not reported
Dyson et al.37 6 (5 M/1 F) Not reported Not reported EVA simulation “EVA” simulations: (1–2 h) designed to mimic geological surveying (hill climbing, flat terrain walking, rock hammer, quiet standing) Mars analog suit 1 g EVA simulation: self-paced

Average VO2: 35.9 ± 0.9 mL/kg/min

HR: 81% of max

Not reported
Standalone Tasks Hill runs: subjects ran up and down 200-m hill 10% until exhaustion (intended as a proxy for traditional treadmill testing) Hill run: pace increased every 2 min until exhaustion Average VO2: 52.4 ± 1.0 mL/kg/min
Rai et al.32 6 (M/F not reported) 23 ± 2.4 Not reported EVA simulation “EVA” simulations: (1–2 h) designed to mimic geological surveying (hill climbing, flat terrain walking, rock hammer, quiet standing) Mars analog suit 1 g EVA simulation: self-paced EVA reached ~85% VO2peak and HRmax Not reported

Data reported as mean ± standard deviation.

M male, F female, VO2 aerobic capacity, VO2pk/VO2max peak or maximal aerobic capacity, RPE rate of perceived exertion, BR bed rest, ex exercise.