FIG. 3.
Viral growth of HIV-1 library in the presence or absence of protease inhibitor. After infection of 5 × 105 MT-2 cells with HIV-1NG-WT (A) or HIV-1NG-PRL (B) (200 ng of p24gag antigen) for 3 h, MT-2 cells were incubated in the absence or in the presence of 0.03, 0.10, or 0.30 μM ritonavir (RTV). The supernatant was stored at −80°C until assay. Infectious virions in the supernatant were evaluated by using a single-round viral infectivity assay. Error bars indicate the standard deviation of three experiments.